 Detergent gel compositions in hexagonal liquid crystal form |
| We claim: 1. A detergent composition comprising a gel wholly or predominantly in hexagonal liquid ... |
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| Process for the manufacture of surfactant cleansing blocks and compositions thereof |
| OF THE INVENTION By way of definition, the term "block" as used herein means formed, solid ... |
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| Solid alkaline detergent and process for making the same |
| Having thus disclosed the present invention, what is claimed is: 1. A process for producing a solid ... |
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| Built synthetic organic detergent composition patties and processes for washing laundry therewith |
| What is claimed is: 1. A pre-measured form-retaining built synthetic organic detergent composition ... |
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| Formation of high active detergent particles |
| What is claimed is: 1. A process for producing high active detergent particles, comprising the ... |
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| Solid cleaner |
| We claim: 1. A solid cast cleaning composition consisting essentially of: (a) an amine oxide ... |
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| Cationic compositions containing diol and/or diol alkoxylate |
| OF THE INVENTION The compounds of the aforementioned formula (1), sometimes referred to herein as ... |
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| Method and sporicidal compositions for synergistic disinfection or sterilization |
| What is claimed is: 1. A method of disinfecting and sanitizing fowl eggs comprising contacting said ... |
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| Means for killing bacterial spores with glutaraldehyde sporicidal compositions |
| 1. A process for killing bacterial spores on instruments, appliances, eating utensils, walls, ... |
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| Glutaraldehyde sterilizing compositions |
| What I claim is: 1. A method of killing dormant spores, which consists of contacting said spores ... |
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Extraction of poly(.beta.-hydroxy butyric acid)
| Details |
Inventors: Walker, John; Whitton, Jonathan R.;
Assignee: Imperial Chemical Industries PLC (London, GB2)
Primary Examiner: Nielsen; Earl A.
Assistant Examiner:
Attorney, Agent or Firm: Cushman, Darby & Cushman
Poly (.beta.-hydroxy butyric acid), PHB, is extracted from a suspension of bacterial cells by causing the cells to flocculate, by pH modification, optionally with heating, and then extracting the PHB from the flocculated cells with a suitable extraction solvent. Flocculation of the cells renders subsequent separation of the PHB solution from the cell debris more facile. Preferably lipids are extracted from the flocculated cells before contact with the PHB extraction solvent. |
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DETAILED DESCRIPTION We claim: 1.
A process for the separation of poly(.
beta.
-hydroxy butyric acid), PHB, from an aqueous suspension of bacterial cells containing PHB wherein the PHB-containing cells are contacted with an extraction solvent in which PHB is soluble and the solvent having the PHB dissolved therein is separated from the cell debris, characterised in that the bacterial cells in the aqueous suspension are caused to flocculate by the step of reducing the pH of the suspension to a value in the range 2 to 5 by treatment with an acid, in combination with at least one of the steps (i) treatment of the suspension with alkali to increase its pH to a value in the range 8 to 12 before acidification, and (ii) heating the suspension to a temperature within the range 50.
degree.
to 200.
degree.
C.
before or after acidification, and then the flocculated cells are separated from the aqueous medium prior to contact of the cells with the extraction solvent.
2.
A process according to claim 1 characterised in that the cells in the suspension are caused to flocculate by increasing the pH of the suspension to a value in the range 8.
5 to 12, heating by injecting steam under pressure into the suspension, and then acidifying the suspension to a pH in the range 3 to 5.
3.
A process according to claim 2 characterised in that the cell suspension is heated to a temperature in the range 60.
degree.
to 100.
degree.
C.
by the injection of steam.
4.
A process according to claim 1 characterised in that, prior to contact with the extraction solvent, the lipids associated with the bacterial cells are extracted from the flocculated cells by contact with a solvent in which the lipids are soluble but in which PHB is insoluble, and the solvent having the lipids dissolved therein is separated from the flocculated cells.
5.
A process according to claim 4 characterised in that the flocculated cells are contacted with the lipid extraction solvent at a temperature in the range 40.
degree.
to 90.
degree.
C.
6.
A process according to claim 4 characterised in that the lipid extraction solvent is acetone or methanol
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Molecular Biology 
