 Method of conditioning flue gas |
| OF THE PREFERRED EMBODIMENTS The conditioners useful in the present invention are finely divided ... |
|
| Enzyme granules |
| What is claimed is: 1. An enzyme granule comprising a granule core of solid material carrying an ... |
|
| Process for producing yeast cells |
| What we claim is: 1. A process for producing yeast cells which comprises culturing aerobically a ... |
|
| Interestification of fats |
| In accordance with the present invention, there is used a customary animal fat, vegetable oil or ... |
|
| Process for the preparation of cholesterol esterase |
| What is claimed is: 1. Process for obtaining cholesterol esterase from micro-organisms, which ... |
|
| .alpha.-Ketoglutarate dehydrogenase gene |
| What is claimed is: 1. An isolated gene comprising 1) a nucleotide sequence which codes for an ... |
|
| Acidic uricase and process for production thereof |
| What is claimed is: 1. Acidic uricase having an optimum pH in the range of 4.7 to 5.1, and ... |
|
|
Gene encoding endoglycoceramidase
| Details |
Inventors: Izu, Hiroyuki; Kurome, Yoko; Izumi, Yoshiya; Sano, Mutsumi; Kato, Ikunoshin; Ito, Makoto;
Assignee: Takara Shuzo Co., Ltd. (Kyoto-Fu, JP)
Primary Examiner: Wax; Robert A.
Assistant Examiner: Stole; Einar
Attorney, Agent or Firm:
An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, and a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof by gene recombinant technology. |
|
DETAILED DESCRIPTION In order to achieve the above objects, the present inventors conducted intensive studies in an effort to isolate the DNA encoding a polypeptide possessing endoglycoceramidase activity for elucidation of its nucleotide sequence.
As a result, the present inventors at last succeeded in elucidating the entire nucleotide sequence of DNA encoding a polypeptide possessing endoglycoceramidase activity and in producing highly pure endoglycoceramidase by simple and easy procedures by use of gene engineering technology.
Based upon these facts, the present invention has been completed.
In one embodiment, the present invention relates to an isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof.
Specifically, the isolated DNA comprises a DNA sequence selected from the group consisting of (a) to (d): (a) a DNA sequence encoding an amino acid sequence of SEQ ID NO:1 or SEQ ID NO:3, or a fragment thereof; (b) a DNA sequence of SEQ ID NO:2 or SEQ ID NO:4, or a fragment thereof; (c) a DNA sequence encoding an amino acid sequence resulting from deletion, addition, insertion or substitution of one or more amino acids in the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:3, or a fragment thereof; and (d) a DNA sequence capable of hybridizing to any one of (a) to (c) above.
In another embodiment, the present invention relates to a recombinant DNA which comprises the isolated DNA of the present invention, to a vector which comprises the recombinant DNA, and to a cell of a procaryote or eucaryote transformed with the vector.
In another embodiment, the present invention relates to a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, comprising the steps of: (a) culturing the cell of the present invention; and (b) recovering the polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof from the culture obtained in Step (a)
|
|
Molecular Biology 
