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Hydrophilic macroporous three dimensional copolymers of hydroxyalkyl acrylates or methacrylates with crosslinking agents and the method of their manufacturing
| Details |
Inventors: Coupek, Jiri; Filka, Karel; Kocourek, Jan;
Assignee: Ceskoslovenska akademie ved (Prague, CS)
Primary Examiner: Foelak; Morton
Assistant Examiner:
Attorney, Agent or Firm:
The invention pertains to hydrophilic macroporous threedimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates (C.sub.1 -C.sub.4 hydroxyalkyl) with crosslinking monomers which contain covalently attached saccharides via an O-glycosidic bond. The invention further pertains to the method of manufacturing of these copolymers by the direct reaction of the basic three dimensional copolymer containing hydroxyl groups with saccharides or their derivatives in an inert organic solvent under catalysis of HCl, BF.sub.3, or their mixtures at 20.degree.-100.degree. C. Saccharides and their derivatives are selected from the group comprising monosaccharides, oligosaccharides, deoxy sugars, amino saccharides, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides, and the like. The invention also pertains to the application of these macroporous three dimensional copolymers with saccharides as sorbents and materials for the affinity chromatography for isolation of physiologically effective materials which contain free bonding sites for saccharides and are able to form specific complexes with saccharides. |
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DETAILED DESCRIPTION We claim: 1.
Hydrophilic macroporous three dimensional copolymers of hydroxyalkyl acrylates or hydroxyalkyl methacrylates, said hydroxyalkyl containing 1-4 carbon atoms, and containing crosslinking agents in amount of 15 to 50 weight percent selected from the group consisting of ethylene diacrylate and ethylene dimethacrylate, wherein said copolymers contain, after glycosylation reaction at the surface, saccharides or their derivatives, selected from the group consisting of monosaccharides, oligosaccharides, deoxy sugars, amino sugars, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides.
2.
A method for producing the copolymers according to claim 1, wherein the three dimensional copolymers containing hydroxyalkyl groups are reacted with saccharides or their derivatives in an inert organic solvent at a temperature of 20.
degree.
-100.
degree.
C.
under catalysis of an agent selected from the group consisting of HCl, BF.
sub.
3, and their mixtures.
Description:
The invention pertains to hydrophilic macroporous three dimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates with crosslinking comonomers containing covalently bound saccharides and to the method of their manufacturing by the direct reaction of the polymers with saccharides under catalysis of Lewis acids.
Hydrophilic macroporous copolymers of hydroxyalkyl methacrylates and hydroxyalkyl acrylates with alkylene dimethacrylates or alkylene diacrylates prepared by a suspension copolymerization of monomers in the presence of inert organic compounds in an aqueous or organic dispersion medium are known from the Czechoslovak Pat.
No.
150,819, which corresponds to the British Pat.
No.
1,370,477, and from the Czechoslovak Pat.
No.
148,828, which corresponds to the British Pat.
No.
1,331,087.
Their outstanding mechanical properties, spherical shape of particles, and a macroporous structure, allowing to penetrate even considerably large molecules into the pores of carrier, led to the extensive applications of these materials in the gel, ion-exchange, adsorption, and affinity chromatography using water or aqueous solutions as an eluent
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Molecular Biology 
