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 Method, device and kit for measurement of tissue plasminogen activator activity

Details
Inventors: Sasamata, Miho; Ito, Katsuhisa; Katoh, Masao; Yano, Shinya; Matsumura, Eriko; Ezoe, Hisanori;
Assignee:
Primary Examiner: Saunders; David
Assistant Examiner:
Attorney, Agent or Firm:

A true activity of tissue plasminogen activator is determined by reacting a solid phase having bound thereto anti tissue plasminogen activator antibody IgG fraction having affinity to a site other than the active site of tissue plasminogen activator with tissue plasminogen activator in a sample and then measuring an activity of the reaction product, preferably in terms of an amount of a substance released from a chromogenic peptide substrate. This measurement enables to use of whole blood or diluted blood as the sample and achieves high sensitivity and high accuracy in a short period of time.

DETAILED DESCRIPTION Namely, the present invention is characterized by reacting, a solid phase having bound thereto anti t-PA antibody IgG fraction having affinity to a site other than the active site of t-PA with t-PA in whole blood or diluted blood and then determining accordingly an activity of the product, and an object of the present invention is to provide the method for determination.
Further, the present invention is characterized by containing at least a solid phase having bound thereto anti t-PA antibody IgG fraction having affinity to a site other than active site of t-PA and reagents for determining an activity of the reaction product between the IgG fraction bound to the solid phase and t-PA in whole blood or diluted blood.
Another object of the present invention is to provide a kit for determination.
A further object of the present invention is to provide novel monoclonal antibody which is also used for the measurement method described above.
The present invention is also characterized by immobilizing a coated layer of anti t-PA-antibody IgG fraction having affinity to a site other than the active site of t-PA at one tip of a plastic stick and a still further object of the present invention is to provide such a measurement device.



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