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Method for producing microbial cells and use thereof to produce oxidation products
| Details |
Inventors: Patel, Ramesh N.; Hou, Ching-Tsang; Laskin, Allen I.;
Assignee: Exxon Research and Engineering Co. (Florham Park, NJ)
Primary Examiner: Penland; R. B.
Assistant Examiner:
Attorney, Agent or Firm: Halluin; Albert P.
Disclosed are newly discovered and isolated methylotrophic microorganism strains and their natural and/or artificial mutants which grow well under aerobic conditions in a culture medium in the presence of methane as the major carbon and energy source. The methane-grown microbial cells possess a high content of protein and can be utilized as such as feedstuffs. The methane-grown microbial cells or enzyme preparations thereof are also useful in converting C.sub.1 -C.sub.6 alkanes to alcohols, particularly methane to methanol, C.sub.3 -C.sub.6 alkanes to the corresponding C.sub.3 -C.sub.6 sec. alcohols and methyl ketones, C.sub.3 -C.sub.6 sec. alcohols to the corresponding methyl ketones, cyclic hydrocarbons to cyclic hydrocarbyl alcohols (e.g., cyclohexane to cyclohexanol) C.sub.2 -C.sub.4 alkenes selected from the group consisting of ethylene, propylene, butene-1 and butadiene to 1,2-epoxides, styrene to styrene oxide, and converting other oxidizable substrates to oxidized products. The newly discovered and isolated methylotrophic microorganism strains may be aerobically grown on a plurality of methyl-radical donating carbon-containing compounds, such as methanol, methylamine, methyl formate, methyl carbonate, dimethyl ether, etc., to produce microbial cells or enzyme preparations capable of aerobically converting C.sub.3 -C.sub.6 linear secondary alcohols to the corresponding methyl ketones. |
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DETAILED DESCRIPTION OF THE PRESENT INVENTION The term "microorganism" is used herein in its broadest sense to include not only bacteria, but also yeasts, filamentous fungi, actinomycetes and protozoa.
Preferably, the microorganisms will include bacteria, and more preferably the bacteria capable of oxidizing methane and methyl-radical donating carbon-containing compounds.
The term "enzyme preparation" is used to refer to any composition of matter than exhibits the desired oxygenase or dehydrogenase enzymatic activity.
The term is used to refer, for example, to live whole cells, dried cells, cell extracts and refined and concentrated preparations derived from the cells, especially purified secondary alcohol dehydrogenase and its NAD.
sup.
+ cofactor and metal requirement.
Enzyme preparations may be either in dry or liquid form.
The term also includes the immobilized form of the enzyme, e.
g.
, the whole cells of the methane or methyl-radical-grown microorganisms or enzyme extracts immobilized or bound to an insoluble matrix by covalent chemical linkage, absorption and entrapment of the enzyme within a gel lattice having pores large enough to allow the molecules of the substrate and of the product to pass freely, but small enough to retain the enzyme.
The term "enzyme preparation" also includes enzymes retained within hollow fiber membranes, e.
g.
, as disclosed by Rony, Biotechnology and Bioengineering (1971).
The term "particulate fraction" refers to the enzyme activity in the precipitated or sedimented material when the supernatant after centrifuging broken cells at 10,000.
times.
g.
for 30 minutes is centrifuged for 1 hour at 10,000.
times.
g.
or greater.
The term "increasing the oxidative state of an oxidizable organic substrate" is meant to include incorporating oxygen in an organic compound, such as in epoxidizing olefins and converting alkanes to alcohols or ketones or increasing the oxidative state of oxygen-containing compounds such as converting alcohols to aldehydes and ketones (i.
e.
, a dehydrogenating reaction)
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Molecular Biology 
