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Molecular Biology

Method-for-purifying-microbial-polysaccharides

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Method for purifying microbial polysaccharides

Details

Inventors: Schroeck, Calvin William;
Assignee: The Lubrizol Corporation (Wickliffe, OH)
Primary Examiner: Jones; Raymond N.
Assistant Examiner: Wiseman; Thomas G.
Attorney, Agent or Firm: Adams, Jr.; James W., Pittman; William H.

Microbial polysaccharides such as xanthan gum sometimes form cloudy suspensions rather than clear solutions when dispersed in water because of the presence of microbial cells therein. An aqueous polysaccharide solution (e.g., the fermentation broth) may be clarified by killing remaining microbial cells (e.g., by pasteurization) and conducting a second fermentation in the presence of an easily filterable microorganism capable of consuming said killed cells. The easily filterable microorganism is typically a fungus and preferably a mold such as the Trichoderma sp. molds. Following the second filtration, the cells of the easily filterable microorganism are preferably removed from the mixture, typically by filtration or centrifugation.

DETAILED DESCRIPTION What is claimed is: 1.
A method for removing bacterial cells from an aqueous mixture comprising a polysaccharide produced by bacterial fermentation which comprises the steps of killing said bacterial cells and causing said mixture to undergo a second fermentation with a Trichoderma sp.
mold, said second fermentation being continued for a period of time adequate to effect solubilization of said killed cells.
2.
A method according to claim 1 wherein the bacterial cells removed are Xanthomonas campestris cells.
3.
A method according to claim 2 which includes the step of separating the mold cells from said mixture.
4.
A method according to claim 3 wherein the mold cells are separated by filtration.
5.
A method according to claim 4 wherein the aqueous mixture is the fermentation broth in which the polysaccharide was produced.
6.
A method according to claim 5 wherein the bacterial cells are killed by heating said mixture to at least about 60.
degree.
C.
7.
A method according to claim 3 wherein the mold cells are separated by centrifugation.
8.
A method according to claim 7 wherein the aqueous mixture is the fermentation broth in which the polysaccharide was produced.
9.
A method according to claim 8 wherein the bacterial cells are killed by heating said mixture to at least about 60.
degree.
C.

Description:
This invention relates to an improved method for clarifying and/or purifying products obtained by microbial fermentation.
More particularly, it relates to a method for removing microbial cells from an aqueous mixture comprising a polysaccharide produced by microbial fermentation which comprises the steps of killing said microbial cells and causing said mixture to undergo a second fermentation in the presence of an easily separable microorganism capable of solubilizing said killed cells.
The microbial production of useful chemicals by fermentation of organic compounds (especially carbohydrates such as sugar and starch) in the presence of a suitable microorganism is a well known procedure

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