 Proteinaceous food product |
| We claim: 1. A method of making an edible proteinaceous food product comprising the steps of: ... |
|
| Process for recovering L-amino acids from fermentation liquors containing them |
| Accordingly, it is an object of the present invention to develop a technique capable of removing ... |
|
| Process for preparing an inhibiting agent against proliferation of animals malignant tumor cells |
| What we claim is: 1. A process for preparing an inhibiting agent against the proliferation of human ... |
|
| Human lymphoblastold cell line and hybridomas derived therefrom |
| We claim: 1. A human lymphoblastoid B cell of the line LTR228 and progeny thereof. 2. A ouabain-... |
|
| Taxol production by taxomyces andreanae |
| Accordingly, it is an object of the present invention to provide an improved process for producing ... |
|
| Intronic overexpression vectors |
| What is claimed is: 1. A non-naturally occurring DNA construct, said DNA construct comprising an ... |
|
| CKS method of protein synthesis |
| This invention is a process for making proteins where a fusion protein of an E. coli enzyme, CKS (CT... |
|
| Direct bonding bracket positioning and mounting tool |
| What is claimed is: 1. An orthodontic tool particularly for positioning and holding a direct ... |
|
| High phosphate process for making low calcium, smooth flow xanthan gum |
| Aqueous compositions of xanthan gum tend to have a "chunky" or non-uniform flow characteristic. A "... |
|
|
Method of controlling the regioselectivity of glycosidic bonds
| Details |
Inventors: Nilsson, Kurt G. I.;
Assignee: Svenska Sockerfabriks AB (Malmo, SE)
Primary Examiner: Wax; Robert
Assistant Examiner: Pratt; Mary E.
Attorney, Agent or Firm: Beveridge, DeGrandi & Weilacher
A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the cabohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, the .alpha. or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligosaccharide compound being separated from the reaction mixture. |
|
DETAILED DESCRIPTION I claim: 1.
A method of controlling the regioselectivity of the glycosidic bond formed between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the carbohydrate part in a glycoconjugate, synthesized by reverse hydrolysis or transglycosidation, comprising reacting a donor substance which is a monosaccharide or oligosaccharide or a glycoside of a monosaccharide or oligosaccharide, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, in the presence of an exo- or endoglycosidase of EC group 3.
2, the .
alpha.
- or .
beta.
-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and separating the oligosaccharide compound from the reaction mixture.
2.
A method as claimed in claim 1, wherein the carbohydrate portion of the donor and acceptor substance includes one or more of the monosaccharides L-fucose, D-galactose, D-mannose, N-acetyl neuraminic acid, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine.
3.
A method as claimed in claim 1, wherein the acceptor substance includes an analog of any of the monosaccharides L-fucose, D-galactose, D-mannose, N-acetyl-D-galactosamine and N-acetyl-D-glucosamine.
4.
A method as claimed in claim 1 wherein the aglycon is an aliphatic or aromatic substance.
5.
A method as claimed in claim 4, wherein the aglycon is a glycosidically bonded methyl, CH.
sub.
3 (CH.
sub.
2).
sub.
n where n=.
gtoreq.
1, phenyl, p-nitrophenyl, o-nitrophenyl, 2-bromoethyl, trimethylsilyl ethyl or an CH.
sub.
2 .
dbd.
C(CH.
sub.
3)--C(O)--OCH.
sub.
2 CH.
sub.
2 group.
6.
A method as claimed in claim 1 wherein the aglycon is a fluorogenic substance.
7.
A method as claimed in claim 1 wherein the aglycon is an amino, nitrile or amide group or contains such a group
|
|
Molecular Biology 
