Method of cultivating Methylomonas probus on methanol containing medium

DETAILED DESCRIPTION OF THE INVENTION The method of this invention comprises the steps of culturing and propagating Methylomonas probus in a medium containing inorganic nutrients and methanol as a carbon source, and then separating and collecting the propagated microbial cells.
Methylomonas probus used in this invention has the following mycological properties.
a.
Morphological properties
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(1) Shape and size of cells:
A single or 2 to 3 chain bacillus
with a size of 0.
5-0.
7 .
times.
1.
0-1.
5.
mu.
.

(2) Polymorphism of cells:
Has no polymorphism.

(3) Mobility: Has mobility with single polar
flagellum.

(4) Spore: Non-sporiferous.

(5) Gram stainning:
Gram negative.

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Examination of the above morphological properties was made on the cells which were cultured in an inorganic salt synthetic medium (1) (the composition of the medium being shown later) at 30.
degree.
C for 24 to 48 hours.

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(6) Acid fastness: - (minus)
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b.
Condition of growth in culture media (1) Agar plate culture with inorganic salt synthetic medium (1): The bacterium of this invention grows abundant and forms smooth, entire and convex circular colonies.
The colonies are semi-transparent and have luster.
The color is grayish white or pink.
(2) Agar slant culture with inorganic salt synthetic medium (1): Growth of the bacterium is of a medium degree.
The formed colony is filiform and has luster.
The color is grayish white or pink.
No diffusive pigment is produced.
(3) Bouillon agar plate culture: No growth or only a very slight degree of growth takes place.
Where a slight degree of growth takes place, the formed colony is less than 0.
5 mm in size (in 48-hour cultivation at 30.
degree.
C).
It is smooth circular, lustrous and transparent