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Method of preparing galcatooligosaccharide
| Details |
Inventors: Matsumoto, Keisuke; Kobayashi, Yoichi; Kan, Tatsuhiko;
Assignee: Kabushiki Kaisha Yakult Honsha (Tokyo, JP)
Primary Examiner: Lilling; Herbert J.
Assistant Examiner:
Attorney, Agent or Firm: Oblon, Spivak, McClelland, Maier & Neustadt
A method of preparing a mixture of galactooligosaccharides having the following formula: Gal-(Gal)n-Glc where Gal represents a galactose residue, Glc represents a glucose residue, and n represents an integer between 1 and 4, and monosaccharides by having microorganisms containing .beta.-galactosidase or .beta.-galactosidase act on lactose, the method comprising: having glucose isomerase coexist in a liquid to be reacted or adding glucose isomerase after completion of the reaction, whereby a portion of glucose prepared by the .beta.-galactosidase processing is converted into fructose. Then, sweetener is prepared by separating monosaccharides from the mixture. |
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DETAILED DESCRIPTION OF THE INVENTION According to the present invention, .
beta.
-galactosidase having a galactosyl transfer reaction activity is utilized to serve as enzyme to hydrolyze lactose.
As a result, glucose and galactose are prepared from lactose, and galactooligosaccharides expressed by a general formula Gal-(Gal)n-Glc (where Gal represents a galactose residue, Glc represents a glucose residue, and n represents an integer ranged between 1 and 4) are also prepared.
The .
beta.
-galactosidase needs to be of a type having strong transfer activity so as to convert, at a high rate, galactose residue of lactose into galactooligosaccharides, the high rate conversion preventing generation of free galactose.
Simultaneously with or after completion of the reaction using .
beta.
-galactosidase, glucose isomerase is allowed to act on glucose prepared by the above-described reaction using the .
beta.
-galactosidase so as to convert a portion of the glucose into fructose.
According to the present invention, since a large portion of the galactose residue of raw material lactose can be converted into galactooligosaccharides, the obtained product displays a high rate of galactooligosaccharide content and monosaccharides which are secondarily prepared contain reduced quantities of galactose, the monosaccharides being therefore mostly composed of glucose and fructose.
The method of preparing galactooligosaccharides according to the present invention is preferably arranged such that glucose isomerase coexists in the liquid to be reacted when galactooligosaccharides are prepared by having microorganisms containing .
beta.
-galactosidase or .
beta.
-galactosidase act on lactose.
The thus coexisting glucose isomerase in the liquid to be reacted according to the method of the present invention successively isomerizes glucose prepared due to the hydrolyzing of lactose so as to convert it into fructose.
As a result, the transfer reaction is protected from an interruption by accumulated glucose in the liquid to be reacted
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Molecular Biology 
