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Method of producing carbon source for fermentation
| Details |
Inventors: Asai, Soichiro; Matsuishi, Tsutomu; Matsushita, Katsunobu; Ikeda, Shigeho; Kobayashi, Kaetsu; Maruyama, Hiromasa;
Assignee: Ajinomoto Co., Inc. (Tokyo, JA)
Primary Examiner: Shapiro; Lionel M.
Assistant Examiner:
Attorney, Agent or Firm: Oblon, Fisher, Spivak, McClelland & Maier
A hydrolyzate of molasses, containing both glucose and fructose, is mixed with calcium hydroxide, and a fructose addition precipitate is recovered. The residual liquor is neutralized, calcium ions are removed, and then the liquor is used as a carbon source for glutamic acid or lysine fermentation. The present method may supply very inexpensive fructose to the market. |
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DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Molasses applicable to the present invention include cane molasses and beet molasses.
Hydrolysis of sucrose in the molasses may be carried out by known methods, such as by the use of mineral acid or an enzyme.
For example, if the pH of molasses is adjusted to 1.
5 - 2 with hydrochloric acid and is heated to 60.
degree.
- 100.
degree.
C for 0.
5 - 4 hours, most of the sucrose in the molasses may be hydrolyzed to hexose, i.
e.
, glucose and fructose.
When molasses is hydrolyzed with mineral acid, the hydrolyzate is neutralized with an alkali, such as calcium hydroxide or sodium hydroxide.
Then, the neutral hydrolyzate is mixed with calcium hydroxide, preferably in an amount of 0.
7 - 1.
5 times the molar quantity of the hexose.
Instead of calcium hydroxide, calcium oxide may be employed.
In this instance, calcium oxide is first converted to calcium hydroxide in the hydrolyzate, and then reacted with fructose.
The mixing must be carried out cautiously.
First, the neutral hydrolyzate is cooled to below 10.
degree.
C, preferably below 5.
degree.
C, and calcium hydroxide, in an amount of 1.
2 - 1.
6 times the molar quantity of the fructose, is added to the cold hydrolyzate.
Then, the seed adducts of fructose and calcium hydroxide are preferably added, and the seeded mixture is aged for 15 - 60 minutes with moderate stirring.
The adduct crystals may also crystallize without seeding.
The remaining calcium hydroxide, is added gradually over a period of about 1 - 2 hours.
During the reaction of calcium hydroxide with fructose, the temperature of the reactant is preferably kept below 5.
degree.
C in order to decrease the decomposition of fructose and glucose.
The above procedure produces large crystals of the adduct which are especially suitable for separation on an industrial scale.
Unless the mixing is carried out with caution, the resulting slurry will be creamy and will consist of mainly fine crystals.
Accordingly, separation of crystals on an industrial scale will be difficult
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Molecular Biology 
