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Method of producing microbial cells from methane
| Details |
Inventors: Morinaga, Yasushi; Yamanaka, Shigeru; Hirose, Yoshio;
Assignee: Ajinomoto Co., Inc. (Tokyo, JA)
Primary Examiner: Shapiro; Lionel M.
Assistant Examiner: Penland; R. B.
Attorney, Agent or Firm: Cooper, Dunham, Clark, Griffin & Moran
A novel strain belonging to genus Methylomonas grows well on a culture medium in the presence of methane as the main carbon source. The microbial cells are useful as a feedstuff. |
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DETAILED DESCRIPTION What is claimed is: 1.
A method which comprises culturing Methylomonas sp.
FERM P-2400 in an aqueous culture medium containing an assimilable nitrogen source and inorganic salts, in the presence of methane as the major carbon source, until the cells of said strain multiply in said culture medium, and recovering the multiplied cells from said medium.
2.
A method as in claim 1, wherein said methane constitutes the sole significant source of assimilable carbon in said medium.
3.
A method as in claim 1 including the addition of from 1% to 5% by weight of carbon dioxide.
4.
A method as in claim 1 carried out in a batchwise manner.
5.
A method as in claim 1 carried out in a continuous manner.
6.
A method as in claim 1 wherein yeast extracts or peptone are utilized as an additional carbon source.
Description:
BACKGROUND OF THE INVENTION This invention relates to a method of producing microbial cells utilizing methane as the main carbon source.
Methane is one of the most inexpensive carbon sources for microbial growth.
It is known that there are many microorganisms capable of growing on a culture medium in the presence of methane as the principle carbon source.
However the growth of the most known strains is quite low and unsatisfactory.
With mixed cultures, superior growth strains are known.
But when the strains are isolated, this superior property of the original strain mixture is somehow lost (Applied Microbiology, 15, 1473-1478 (1967) and 21, 511-515 (1971)).
With pure cultures, superior strains, capable of good growth, are not known.
Since it is difficult to maintain stable mixed cultures during a large number of cycles, the industrial production of single cell protein from methane is a problem.
THE INVENTION It is now been found that a novel strain belonging to genus Methylomonas which was isolated from soil grows well as a pure culture on a medium in the presence of methane as a main carbon source.
The new strain is Methylomonas sp AJ-3670 (FERM P-2400), which has been deposited with the Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry for Industrial Trade and Industry, Chiba, Japan, and is freely available from the Institute
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Molecular Biology 
