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Methods for producing human lymphocytes and human monoclonal antibodies, and human monoclonal antibodies produced thereby
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Inventors: Kudo, Toshio; Hiyamuta, Shuichi; Tanedani, Toshiyuki; Kadota, Akihiko;
Assignee: Idemitsu Kosan Company Limited (Tokyo, JP)
Primary Examiner: Scheiner; Toni R.
Assistant Examiner:
Attorney, Agent or Firm: Birch, Stewart, Kolasch & Birch, LLP
A method for isolating a human lymphocyte producing an antibody specific to a desired antigen, a method for isolating a monoclonal antibody, as well as monoclonal antibodies produced by the method, are described. The present invention provides a method for isolating a human lymphocyte producing an antibody comprising, in the order mentioned, the steps of: transplanting human lymphocytes into an immunodeficient animal so that the human lymphocytes take in the animal body; immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen; recovering lymphocytes producing antibody by incubating with anti-mouse antibody; and separating human lymphocytes from mouse by centrifugation. The present invention also provides a method for producing a monoclonal antibody comprising, in the order mentioned, the steps of: immortalizing the human lymphocytes producing the antibody which were obtained by the above-mentioned method of the present invention; cloning the obtained immortalized human-derived lymphocytes producing the antibody; and recovering a monoclonal antibody specific to the desired antigen from the cloned immortalized human-derived lymphocytes. |
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DETAILED DESCRIPTION Accordingly, an object of the present invention is to provide a method for producing a human lymphocyte which produces an antibody, especially IgG antibody, which is specific to a desired antigen.
Another object of the present invention is to provide a method for producing human monoclonal antibodies using the lymphocyte produced by the method of the present invention.
Still another object of the present invention is to provide human monoclonal antibodies useful for treatment or diagnosis of cancers.
The present inventors intensively studied to find that human lymphocytes which mainly produce IgG and IgA antibodies can be obtained by transplanting human lymphocytes to an immunodeficient animal so that the human lymphocytes take in the animal body; and immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen, thereby completing the present invention.
That is, the present invention provides a method for producing a human lymphocyte producing an antibody comprising, in the order mentioned, the steps of: transplanting human lymphocytes to an immunodeficient animal so that the human lymphocytes take in the animal body; immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen; and recovering the human lymphocytes producing the antibody from the animal.
The present invention also provides a method for producing a monoclonal antibody comprising, in the order mentioned, the steps of: immortalizing the human lymphocytes producing the antibody, which were obtained by the above-mentioned method of the present invention; cloning the obtained immortalized human-originated lymphocytes producing the antibody; and recovering a monoclonal antibody specific to the desired antigen from the cloned immortalized human-originated lymphocytes.
The present invention still further provides a human monoclonal antibody which specifically reacts with the extracellular domain of human c-erbB2 cancer gene product
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Molecular Biology 
