 Polymers of N,N-disubstituted acrylamides and method for the preparation thereof |
| What is claimed is: 1. A block copolymer, the block copolymer being of the configuration AB or ABA, ... |
|
| Derivatives of 5-hydroxy-6-diloweralkylaminomethyl-benzofurans |
| We claim: 1. A compound of the formula: ##SPC5## wherein R is lower alkyl, R' is selected from the ... |
|
| Process for producing parasiticidal milbemycin derivatives by culturing streptomyces |
| We claim: 1. A process for the production of a compound of formula (I) ##STR10## wherein R.sup.1 is ... |
|
| Hypocholesterolemic fermentation products |
| What is claimed is: 1. A compound of structural formula I in substantially pure form: ##STR6## 2. A... |
|
| Contact lens disinfecting and preserving solution |
| The embodiments of the invention in which an exclusive property or privilege is claimed are defined ... |
|
|
Nondenaturing zwitterionic detergents
| Details |
Inventors: Hjelmeland, Leonard M.;
Assignee: The United States of America as represented by the Secretary of the (Washington, DC)
Primary Examiner: Roberts; Elbert L.
Assistant Examiner:
Attorney, Agent or Firm: Roberts, Jr.; John S.
A nondenaturing zwitterionic detergent for proteins which, for example, consists of an effective amount of 3-[(3-chloamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). This detergent is of extreme interest in the biological study of proteins due to its nondenaturing characteristic. Other examples of the group may be prepared from different alicyclic compounds, for example, utilizing cholic acid and in others deoxycholic acid and dehydroabietic acid. A process for the preparation of these compounds starts with cholic or the equivalent and from this is prepared the triethylammonium salt in tetrahydrofuran (THF). After the salt is completely dissolved in THF, ethyl chloroformate is added and the flask cooled to 0.degree. C. Then the mixed anhydride which forms is reacted with dimethylaminopropylamine to form the dimethylaminopropyl derivative of a carboxylic acid amide. Finally, the tertiary amine group is reacted with propanesultone to give the sulfobetaine product. An improved procedure for preparation of these compounds and especially for the last step (as for CHAPSO) to react the N-(3-dimethylaminopropyl)cholamide with sodium-1-chloro-2-hydroxy-3-propanesulfonate. |
|
DETAILED DESCRIPTION I claim: 1.
A nondenaturing zwitterionic detergent for protein consisting of an effective amount of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate in a water carrier.
2.
The process according to claim 1 of producing 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate.
3.
The compound 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate.
4.
The process according to claim 1 of producing 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate.
5.
The compound 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate.
6.
A process for producing a nondenaturing zwitterionic detergent for protein derived from the reaction of an acid selected from one member of a group of carboxylic acids consisting of cholic acid, deoxycholic acid, and dehydroabietic acid reacted with a polyethyleneamine selected from one member of the group consisting of ##STR8## or reacted with a polypropyleneamine selected from one member of the group consisting of ##STR9## and reacted with an alkylating agent selected from one member of the group consisting of propanesultone, butanesultone, sodium 3-Cl,2-OH-propanesulfonate, sodium 2-Br-ethane sulfonate, and sodium chloroacetate wherein (1) said carboxylic acid is reacted with an amine to form a triethylammonium salt in the solvent tetrahydrofuran; (2) after the salt is completely dissolved, ethyl chloroformate is added and the flask is cooled to 0.
degree.
C.
and a precipitate forms which is triethylamine hydrochloride which is filtered away from the mixed anhydride; (3) the product, mixed anhydride, reacts with a polyamine to form the polyamine derivative of a carboxylic acid amide as well as ethanol and carbon dioxide by products; (4) the polyamine is reacted with an alkylating agent selected from one member of the group consisting of ##STR10## to give the betaine.
Description:
This invention relates to a nondenaturing zwitterionic detergent for proteins which, for example, consists of an effective amount of 3-[(3-chloamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)
|
| Related patents |
|
|
Method of producing high molecular derivatives
What we claim is: 1. A method for producing high molecular weight derivatives containing at least a recurring unit of the formula: ##SPC8## wherein R means an organic ...
|
|
|
Polyvinyl quaternaries
We claim: 1. The process for preparing polyvinyl quaternary amines which comprises reacting an aqueous solution of a tertiary amine with ethylene dihalide in the mol ...
|
|
|
Process for preparing aryl- or heteroarylhexadienoic acids
We claim: 1. A process for preparing aryl- or heteroarylhexadienoic acids, characterized in that an aryl- or heteroarylacetylene of formula: R--C.tbd.CH (I), wherein R ...
|
|
|
Developing method for developer transfer under electrical bias and apparatus therefor
The present invention has been made to eliminate the above-noted problems peculiar to the various developing methods using one-component developer, and it is a primary ...
|
|
|
Photosensitive composition with polymer having diazonium salt in side chain
OF THE INVENTION A preferred compound of formula (I) is such that B represents a substituted aralkylene group, C is --O--, l=0, and m=n=1. Also preferred is a compound ...
|
|
|
Silyl ethers, a process for their production and their use as polymerization initiators
We claim: 1. Silyl ethers corresponding to the formula: ##STR7## in which R.sup.1 and R.sup.2 represent phenyl, tolyl, p-tert.-butyl phenyl, o- and p-chlorophenyl, 2,4-...
|
|
|
Process for nonaqueous dispersion polymerization of butadiene in the presence of polymeric dispersing agents
Butadiene can be polymerized to a very high (95% or greater) cis-1,4-polybutadiene in a nonaqueous liquid dispersion medium containing random copolymers of butadiene ...
|
|
|
Developer compositions containing quaternized vinylpyridine polymers, and copolymers
What is claimed is: 1. A positively charged dry developer composition comprised of resin particles, pigment particles, carrier particles, and a charge enhancing ...
|
|
|
Imaging element and process with laser absorptive ink barrier layer
The present invention is directed to a method for forming an image and an imaging element for use in said method. The method comprises an imagewise exposure of an ...
|
|
|
Process and catalyst for olefin polymerization
OF THE INVENTION The organophosphates and organophosphoramides contemplated for use in the present invention are those which are capable of causing the metal halide to ...
|
|
|
Molecular Biology 
