Novel bioconverting microorganisms

DETAILED DESCRIPTION OF THE INVENTION This invention relates to two Actinoplanes missouriensis strains which together co-synthesize a new glycopeptide antibiotic and which independently are useful in certain bioconversion reactions.
More particularly, the Actinoplanes missouriensis strains of this invention have the ability to co-synthesize the formula 1 glycopeptide antibiotic which has been designated CUC/CSV.
The formula 1 compound is the subject of a co-pending application of LaVerne D.
Boeck, Gladys M.
Clem, Charles L.
Hershberger, Marie T.
Anderson and Karl H.
Michel entitled GLYCOPEPTIDE COMPOUND CUC/CSV AND PROCESS FOR ITS PRODUCTION, Ser.
No.
544,338, now U.
S.
Pat.
No.
4,537,715, filed herewith this even date.
The novel Actinoplanes missouriensis strains of this invention have been arbitrarily designated CUC 014 and CSV 558.
Cultures CSV 558 and CUC 014 are strains obtained by chemical mutagenesis of Actinoplanes missouriensis ATCC 31683, a strain which produces the glycopeptide antibiotic actaplanin.
A.
missouriensis ATCC 31683 in turn was derived by a series of mutations from the wild type A.
missouriensis ATCC 23342 strain.
The glycopeptide antibiotic actaplanin (which is also known as antibiotic A4696) and the A.
missouriensis strain ATCC 23342 are described by Hamill et al.
in U.
S.
Pat.
Nos.
3,952,095 and 4,115,552.
The actaplanin-producing A.
missouriensis strains ATCC 32680, ATCC 31682 and ATCC 31683 are described by Debono et al.
in U.
S.
Pat.
Nos.
4,322,406 and 4,375,513.
The novel A.
missouriensis strains CSV 558 and CUC 014 of this invention do not produce actaplanin in any measurable quantity.
Instead, strain CUC 014 secretes an intermediate which is then converted by strain CSV 558 to give the formula 1 compound.
CHARACTERIZATION OF CULTURES CSV 558 AND CUC 014 The following taxonomic study of the new CSV 558 and CUC 014 cultures were made together with the parent ATCC 31683 and wild type ATCC 23342 strains in order to provide a complete evaluation.
Cultures CSV 558 and CUC 014 are also classified as strains of Actinoplanes missouriensis