Preparation of antibiotic LL-D05139.beta. from cultures of Glycomyces harbinensis, gen. nov., sp. nov.

DETAILED DESCRIPTION OF THE INVENTION The new antibacterial agent designated LL-D05139.
beta.
is formed during the cultivation under controlled conditions of a new strain of a new genus and species named Glycomyces harbinensis, gen.
nov.
, sp.
nov.
Depending on the fermentation conditions, variable amount of azaserine is coproduced.
Under proper cultivation conditions, using defined medium LL-D05139.
beta.
is produced as the major active component.
The physiochemical characteristics of LL-D05139.
beta.
are as follows: 1.
Molecular weight: 244 (FAB-MS).
2.
UV.
sub.
max at 250 nm, E.
sub.
1cm.
sup.
1% =646 (UV spectrum as shown in FIG.
I, water solution).
3.
IR spectrum (KBr disc) as shown in FIG.
II.
4.
Optical Rotation: [.
alpha.
].
sub.
D.
sup.
26 =+57.
degree.
.
+-.
5.
degree.
(C,0.
19%, water).
5.
.
sup.
1 H NMR Spectrum (D.
sub.
2 O, ppm from TMS) as shown in FIG.
III, 1.
56(3H, d, J=7.
4 Hz), 4.
07(1 H, q.
J=7.
4 Hz), 4.
49(2-3H, m), 5.
19(1H, broad s).
6.
Decomposes rapidly below pH 5.
0, stable in weakly basic solutions.
Acid hydrolysis of LL-D05139 releases one molar equivalent each of L-alanine and L-serine as determined by standard amino acid analysis.
Both alanine and serine derived from LL-D05139 have the L-configuration as determined by the well-accepted gas chromatography procedure using n-Lauroyl-L-valine-t-butylamide as stationary phase.
See R.
Charles et al.
, J.
Chromatography, 112: 121-133 (1975).
N-Terminal analysis of LL-D05139.
beta.
using the Edman degradation procedure followed by HPLC analysis of the PTH-amino acid identified the N-terminal amino acid to be L-alanine.
Therefore this agent has been assigned the following structure: ##STR4## This new antibiotic producing strain was isolated from a soil sample collected in Harbin, China and is maintained in the culture collection of the Lederle Laboratories Division, American Cyanamid Company, Pearl River, N.
Y.
as culture number LL-D05139.
A viable culture of this new microorganism has been deposited with the Culture Collection Laboratory, Northern Utilization Research and Development Division, U