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| Production of a recombinant human colony stimulating factor |
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| Methyl isocyanate process |
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| Sulphonic acid salts of S-adenosilmethionine |
| I claim: 1. Stable salts of S-adenosil-L-methionine (SAM) with sulphonic acids selected from the ... |
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| Fortimicin AK |
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| 3-Cyano or acyl-4-aryl-5-arylazo-6-hydroxypyridone-2 dyes |
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| Semi-rigid polyurethane foam used in packaging |
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Process for making L-phenyl acetyl carbinol (PAC), microorganisms for use in the process, and a method of preparing the microorganisms
| Details |
Inventors: Seely, Robert J.; Heefner, Donald L.; Hageman, Robert V.; Yarus, Michael J.; Sullivan, Sally A.;
Assignee: Synergen Associates, Inc. (Boulder, CO)
Primary Examiner: Lilling; Herbert J.
Assistant Examiner:
Attorney, Agent or Firm: Finnegan, Henderson, Farabow, Garrett & Dunner
Mutations are induced in a microorganism selected from the species Saccharomyces cerevisiae or from the species Candida flareri. The resulting mutants are cultured in the presence of a fermentation inhibitor, such as acetaldehyde, ephedrine or PAC-dione, to form colonies having resistance to the inhibitor. Cells from the colonies are isolated and tested for yield of phenyl acetyl carbinol (PAC) in a fermentation with benzaldehyde and pyruvate. Yeast cells from the colonies that produce elevated levels of PAC are selected for use in subsequent fermentations. PAC is useful as an intermediate in the preparation of l-ephedrine and d-pseudoephedrine, two well-known medicinal chemicals. |
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DETAILED DESCRIPTION This invention aids in fulfilling these needs in the art by providing an improved process for the production of PAC by yeast transformation of benzaldehyde.
The process of this invention makes it possible to obtain higher yeast productivity and higher concentrations of PAC in the fermentation liquor than in present processes.
More particularly, this invention provides a process for the production of L-phenyl acetyl carbinol (PAC) wherein the process comprises providing a submerged culture of a microorganism in an aqueous fermentation medium containing assimilable carbon.
The organism is cultivated under anaerobic or oxygen-limited conditions in the presence of benzaldehyde and pyruvate to form PAC in a concentration of at least about 1.
0 g/L in the fermentation medium.
The organism is a mutant of a parent strain of Saccharomyces cerevisiae or a mutant of a parent strain of Candida flareri.
The mutant is more resistant to aldehyde inhibition, produces less acetaldehyde and provides a higher concentration of PAC in the fermentation medium than a corresponding fermentation using the parent strain under otherwise identical conditions.
The PAC can be separated from the fermentation medium.
This invention also provides a method for producing the mutant microorganisms.
The method comprises providing a microorganism capable of producing a measurable amount of L-phenyl acetyl carbinol (PAC) when cultured with benzaldehyde and pyruvate in an aqueous medium, wherein the organism is selected from species of Saccharomyces cerevisiae or from species of Candida flareri.
The organism is chemically mutagenized with a chemical mutagen or by irradiation.
The resulting mutated organisms are cultured in the presence of acetaldehyde under conditions to form colonies having resistance to acetaldehyde.
Yeast cells are isolated from the colonies and tested for yield of L-phenyl acetyl carbinol in a fermentation with benzaldehyde and pyruvate.
Cells from colonies producing elevated levels of PAC can be employed in large scale fermentation
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Molecular Biology 
