Process for measuring the plasminogen content of a sample

DETAILED DESCRIPTION What I claim is: 1.
The process of measuring the plasminogen content of a plasma sample comprising the steps of: a.
eliminating any antiplasmin from the plasma sample, b.
adding a predetermined amount of fibrinogen to the sample, c.
adding an amount of plasminogen activator sufficient to activate all possible plasminogen present in the sample, and d.
incubating the sample a sufficient period of time to form a solution of fibrinogen and antiplasmin-free, plasminogen-activated plasma, e.
adding thrombin to the solution, and f.
timing the reaction until the moment of fibrin coagulation is observed in the solution, the plasminogen content to be established by comparing the observed time to fibrin coagulation with an established normal standard.
2.
The process of measuring the plasminogen content of a plasma sample comprising the steps of: a.
eliminating any antiplasmin from the plasma sample, b.
adding a predetermined amount of fibrinogen to the sample, c.
selecting a plasminogen activator from the group consisting of urokinase and streptokinase, d.
adding an amount of the selected plasminogen activator sufficient to activate all possible plasminogen present in the sample, e.
incubating the sample a sufficient period of time to form a solution of fibrinogen and antiplasmin-free, plasminogen-activated plasma, f.
adding thrombin to the solution, and g.
timing the reaction until the moment of fibrin coagulation is observed in the solution, the plasminogen content to be established by comparing the observed time to fibrin coagulation with an established normal standard.
3.
The process of claim 2 wherein the antiplasmins are removed from the plasma sample in step (a.
) by washing the sample with isoamylalcohol.
4.
The process of claim 2 wherein the plasminogen activator used in step (c.
) is urokinase.
5.
The process of claim 2 wherein the plasminogen activator used in step (c.
) is streptokinase.
6.
The process of claim 5 wherein the streptokinase is added to the fibrinogen of step (b