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Process for obtaining glucose-isomerase
| Details |
Inventors: LLorente Agudo, Nieves; Elia Miguel, Maria F.; Aranguren Escobar, Maria A.; Pares Olivet, Eulalia; Fernandez Garvajosa, Jose M.;
Assignee: Compania Espanola de Petroleos, S.A. (ES)
Primary Examiner: Shapiro; Lionel M.
Assistant Examiner:
Attorney, Agent or Firm: Wenderoth, Lind & Ponack
There is provided a process for producing glucose-isomerase in high yield by culturing under aerobic conditions, the strain Streptomyces griseoflavus (NCIB 11542). |
|
DETAILED DESCRIPTION We claim: 1.
Process for producing glucose-isomerase, which comprises culturing continuously or discontinuously, under aerobic conditions, a strain of the species Streptomyces griseoflavus A-40 (NCIB-11542) in a culture medium and recovering glucose-isomerase from said medium.
2.
Process for producing glucose-isomerase, as claimed in claim 1 wherein the culture medium has the following composition: soy flour of a concentration ranging from 0.
1% to 10%, and/or tryptone of a concentration ranging from 0.
01% to 2%, and/or xylans of a concentration ranging from 0.
1% to 2%, and further containing an ammonium salt.
3.
Process for producing glucose isomerase according to claim 2 wherein said ammonium salt is ammonium sulphate.
4.
Process for obtaining glucose-isomerase as claimed in claim 1 wherein the cultivation takes place at a temperature ranging between 20.
degree.
C.
and 50.
degree.
C.
, and wherein the pH ranges between 3 and 10.
5.
Process for producing glucose-isomerase according to claim 4 wherein said temperature is between 30.
degree.
C.
and 35.
degree.
C.
and said pH is between 6.
5 and 7.
5.
6.
Process for producing glucose-isomerase as claimed in claim 1 wherein the culture is subjected to agitation at between 50 and 1000 r.
p.
m.
7.
Process for producing glucose-isomerase, as claimed in claim 1 wherein the microorganism has a speed of dilution in continuous culture that ranges between 0.
05 and 1.
2 hours.
sup.
-1.
8.
The process for producing glucose-isomerase according to claim 1 wherein the cultivation time ranges between 17 and 215 hours and the units/gram of cells produced is between 107 and 1000.
9.
The process for producing glucose-isomerase according to claim 9 wherein the cells of said strain are separated from the culture medium and are washed with a buffer comprising imidazole at a concentration of 5.
times.
10.
sup.
-2 molar, hexahydrated cobalt chloride at a concentration of between 10.
sup.
-3 and 10.
sup.
-4 molar and heptahydrated magnesium sulphate at a concentration between 10
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Molecular Biology 
