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Process for preparing an inhibiting agent against proliferation of animals malignant tumor cells
| Details |
Inventors: Tajima, Tomoyuki;
Assignee: Koken Ltd. (Tokyo, JP); Nagasu; Youichiro (Kanagawa, JP)
Primary Examiner:
Assistant Examiner:
Attorney, Agent or Firm:
The present invention relates to a process for preparing an inhibiting agent against the proliferation of malignant tumor cells of animals including human beings by cultivating animal malignant tumor cells, removing said malignant tumor cells from the culture medium and successively isolating a fraction having a molecular weight not less than 500 by fractional filtration. |
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DETAILED DESCRIPTION What we claim is: 1.
A process for preparing an inhibiting agent against the proliferation of human renal carcinoma cells comprising cultivating human renal carcinoma cells from said culture medium, and successively isolating a fraction from the culture medium having a molecular weight not less than 500 by fractional filtration, said fraction inhibiting the proliferation of human renal carcinoma cells.
2.
A process for preparing an inhibiting agent against the proliferation of human renal carcinoma cells according to claim 1, wherein the culture medium is Basal Medium Eagle not containing serum.
Description:
SUMMARY OF THE INVENTION The present invention relates to a process for preparation of an inhibiting agent against the proliferation of malignant tumor cells of animals including human beings.
The substance, which is obtained by cultivating animal malignant tumor cells on a medium and extracting the medium after removing said tumor cells, shows a remarkable inhibiting activity against the proliferation of the malignant tumor cells, although it shows the inhibiting activity somewhat against the proliferation to normal cells, too.
The fractions are prepared by cultivating the malignant tumor cells in the culture medium for extraction (extraction medium) at a temperature between 30.
degree.
C.
and 37.
degree.
C.
, removing said malignant tumor cells, and subjecting the culture medium to filtration by molecular sieving to fractionate at the molecular weight of 500.
For example, the culture medium is filtered through a membrane filter which fractionates at the molecular weight of 500, such as a UM 05 filter or YA 05 filter made by Amicon, such that the culture medium is separated into a fraction having a molecular weight not less than 500 and another fractions having a molecular weight less than 500.
When these inhibiting effects against the proliferation of the cultivated tumor cells were investigated by using each of these fractions, it was determined that the substance isolated from the fraction having a molecular weight not less than 500 showed more remarkable inhibiting activity than the inhibiting activity of the filtrate having a molecular weight less than 500
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Molecular Biology 
