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Process for producing biotin vitamers using novel microorganisms
| Details |
Inventors: Kishimoto, Jiro; Haze, Shinichiro; Ifuku, Ohji;
Assignee: Shiseido Company Ltd. (Tokyo, JP)
Primary Examiner: Lilling; Herbert J.
Assistant Examiner:
Attorney, Agent or Firm: Wegner, Cantor, Mueller & Player
A mutant strain having a glucose consumption rate is at most 1/4 that of wild type strains is provided. The mutant strain belongs to Escherichia, Bacillus, Pseudomonas, or Serratia; has a glucose consumption rate of at most one fourth that of the corresponding wild type strain, and the feedback repression by biotin is removed. Further, a process for producing biotins using this mutant strain is provided. |
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DETAILED DESCRIPTION We claim: 1.
A process for producing biotin vitamers which comprises (a) cultivating in a nutrient medium a mutant strain of E.
coli having (i) a glucose consumption rate of at most 1/4 the rate of the corresponding wild strain, (ii) feedback repression by biotin removed, and operon originating from E.
coli inserted in the vector DNA; and (b) collecting biotin and/or desthiobiotin formed and accumulated in the culture broth; wherein said process is characterized by the total production of desthiobiotin and biotin in an amount at least about 3.
5 times greater than the corresponding parent strain of E.
coli.
2.
A process of claim 1, wherein said mutant strain is a strain selected from the group consisting of DRG 024, DRG 005, DRG 014, DRG 026, and DRG 101.
3.
A process of claim 1, wherein said mutant strain is DRG 024.
4.
A process of claim 1, wherein said mutant strain is DRG 005.
5.
A process of claim 1, wherein said mutant strain is DRG 014.
6.
A process of claim 1, wherein said mutant strain is DRG 026.
7.
A process of claim 1, wherein said mutant strain is DRG 101.
Description:
FIELD OF THE INVENTION The present invention relates to novel mutant strains having a glucose consumption rate which is at most 1/4 that of wild type strains, particularly mutant strains which belongs to the genus Escherichia which are used for producing biotins.
Further, the present invention relates to a process for producing biotin vitamers using these mutant strains.
DESCRIPTION OF THE RELATED ART Various mutant strains have been produced in order to enhance the productivity of a specific useful substance.
For example, in the general view of bacteria used for producing biotin, which is a vitamin important for animals, plants, and microorganisms, as the specific useful substance, those in which artificial mutated microorganisms such as the genus Bacillus, the genus Chromobacterium, the genus Pseudomonas, and the genus Arthrobacter are known.
More specifically, in recent years, a strain which belongs to the genus Escherichia, which has a resistance to
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Molecular Biology 
