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 Process for producing dextrose using mixed immobilized enzymes

Details
Inventors: Thompson, Kenneth N.; Lloyd, Norman E.; Johnson, Richard A.;
Assignee: Standard Brands Incorporated (New York, NY)
Primary Examiner: Jones; Raymond N.
Assistant Examiner: Wiseman; Thomas G.
Attorney, Agent or Firm:

Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.

DETAILED DESCRIPTION What is claimed is: 1.
A process for converting starch to dextrose comprising treating starch with alpha-amylase or acid or a combination thereof to obtain a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch and then treating the partially hydrolyzed starch solution with an enzyme system comprising entrapped glucoamylase within a polymeric matrix and immobilized alpha-amylase selected from the group consisting of alpha-amylase covalently bonded to an insoluble carrier, alpha-amylase adsorbed on an insoluble carrier, and entrapped alpha-amylase under conditions whereby a hydrolysate containing at least about 92% dextrose on an ash free, dry basis is produced.
2.
A process for converting starch to dextrose as defined in claim 1, wherein the alpha-amylase is entrapped in a polymeric matrix.
3.
A process for converting starch to dextrose comprising treating starch with alpha-amylase or acid or a combination thereof to obtain a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch and then treating the partially hydrolyzed starch solution with an enzyme system comprising entrapped alpha-amylase and immobilized glucoamylase selected from the group consisting of glucoamylase covalently bonded to an insoluble carrier and glucoamylase adsorbed on an insoluble carrier.
4.
A process for converting starch to dextrose as defined in claim 3, wherein the alpha-amylase is entrapped in a polymeric matrix.




Description:
THE INVENTION This invention relates to a process for converting starch to dextrose.
More particularly, this invention relates to a process for converting starch to dextrose by the use of an enzyme system comprising immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amyalse, immobilized alpha-amylase and mixtures thereof.
Processes for hydrolyzing starch to dextrose are well known in the art.
These methods can be grouped into two broad categories



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