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Molecular Biology

Process-for-producing-dextrose-using-mixed-immobilized-enzymes

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Process for producing dextrose using mixed immobilized enzymes

Details

Inventors: Thompson, Kenneth N.; Johnson, Richard A.; Lloyd, Norman E.;
Assignee: Standard Brands Incorporated (New York, NY)
Primary Examiner: Monacell; A. Louis
Assistant Examiner:
Attorney, Agent or Firm:

Process for converting starch to dextrose wherein a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch is contacted with an enzyme system under conditions whereby substantially complete conversion of the starch to dextrose is achieved. The enzyme system comprises immobilized glucoamylase and alpha-amylase selected from the group consisting of soluble alpha-amylase, immobilized alpha-amylase and mixtures thereof.

DETAILED DESCRIPTION What is claimed is: 1.
A process for converting starch to dextrose comprising treating starch with alpha-amylase to obtain a partially hydrolyzed starch solution containing at least 10 percent hydrolyzed starch and then treating the partially hydrolyzed starch solution with an enzyme system comprising immobilized glucoamylase selected from the group consisting of glucoamylase covalently bonded to an insoluble carrier and glucoamylase adsorbed on an insoluble carrier and immobilized alpha-amylase selected from the group consisting of alpha-amylase covalently bonded to an insoluble carrier and alpha-amylase adsorbed on an insoluble carrier under conditions whereby a hydrolysate containing at least about 92 percent dextrose on an ash free, dry basis is produced.
2.
A process for converting starch to dextrose as defined in claim 1, wherein the amount of immobilized glucoamylase and the amount of immobilized alpha-amylase are such as to provide a ratio of dextrinizing activity to glucoamylase activity of at least 0.
2 liquefons per glucoamylase unit.
3.
A process for converting starch to dextrose as defined in claim 1, wherein the amount of immobilized glucoamylase and the amount of immobilized alpha-amylase are such as to provide a ratio of dextrinizing activity to glucoamylase activity of at least 1 liquefon per glucoamylase unit.
4.
A process for converting starch to dextrose as defined in claim 1, wherein the amount of immobilized glucoamylase and the amount of immobilized alpha-amylase are such as to provide a ratio of dextrinizing activity to glucoamylase activity of at least 3 liquefons per glucoamylase unit.
5.
A process for converting starch to dextrose as defined in claim 1, wherein the immobilized alpha-amylase is prepared from a soluble alpha-amylase preparation having a S/L value of at least about 3.
6.
A process for converting starch to dextrose as defined in claim 1, wherein the immobilized alpha-amylase is prepared from a soluble alpha-amylase preparation having a S/L value of at least about 50

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