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Process for the production of polysaccharide
| Details |
Inventors: Jarman, Trevor R.; Govan, John R. W.;
Assignee: Talres Development (N.A.) N.V. (AN)
Primary Examiner: Tanenholtz; Alvin E.
Assistant Examiner:
Attorney, Agent or Firm: Ostrolenk, Faber, Gerb & Soffen
A process for the production of polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues, comprises cultivating in a nutrient medium therefor a strain of Pseudomonas, which is non-pathogenic to humans, and which has been obtained by treating a non-mucoid species of Pseudomonas, which is non-pathogenic to humans, with a .beta.-lactam or aminoglycoside antibiotic whereby a mucoid strain tolerant to said antibiotic was selected, and isolating from the medium the polysaccharide produced. |
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DETAILED DESCRIPTION We claim: 1.
A process for the production of polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues, which comprises cultivating in a nutrient medium therefor a strain of Pseudomonas, which is non-pathogenic to humans, and which has been obtained by treating a non-mucoid species of Pseudomonas, which is non-pathogenic to humans, with a .
beta.
-lactam or aminoglycoside antibiotic whereby a mucoid strain tolerant to said antibiotic was selected, and isolating from the medium the polysaccharide produced.
2.
A process according to claim 1, in which the penicillin antibiotic is a carboxyalkyl or carboxyaralkyl derivative of penicillin, an ester thereof or a 3-halophenyl derivative of penicillin.
3.
A process according to claim 2, in which the penicillin antibiotic is carbenicillin.
4.
A process according to claim 1 in which the strain of Pseudomonas has been derived from P.
mendocina, P.
tabaci or P.
putida.
5.
A process according to claim 1, in which the strain used has been obtained by subjecting the mucoid species of Pseudomonas to culture on a selective medium containing a generally inhibitory concentration of the antibiotic.
6.
A process according to claim 5 in which carbenicillin had been included in the selective medium at a concentration of about 600 .
mu.
g/ml.
7.
A process according to claim 5 using P.
mendocina or P.
putida, selected by indicating carbenicillin the selective medium at a concentration of from 500 to 600 .
mu.
g/ml.
Description:
This invention relates to a process for the production of polysaccharide of the alginate type from a microbial source other than Azotobacter vinelandii.
Alginic acid, a hydrophilic colloidal carbohydrate acid, is a variable block copolymer composed of D-mannuronic and L-guluronic acid units.
Alkali salts of alginic acid are soluble in water and one of the outstanding characteristics of such alginate solutions is their high viscosity at low concentrations
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Molecular Biology 
