Production of a recombinant human colony stimulating factor

DETAILED DESCRIPTION What is claimed is: 1.
A process for producing a protein capable of stimulating proliferation of monocytic cells in both human and mouse bone marrow assays comprising culturing host cells containing a DNA vector which contains a DNA sequence encoding the polypeptide sequence encompassing the mature form of the protein of FIG.
2, the DNA sequence being in operative association with an expression control sequence and recovering the protein so produced from the cultured host cells.
2.
The process according to claim 1, wherein said cells are a mammalian cell line.
3.
The process according to claim 2, wherein said cells are selected from the group consisting of a primate cell line, and a rodent cell line.
4.
The process according to claim 1, wherein said vector is p3ACSF-69.
5.
The process of claim 1, wherein the DNA is cDNA.




Description:
The present invention relates to a process for producing a recombinant protein, named CSF-69.
More specifically, this invention relates to a method employing a novel DNA sequence and recombinant DNA molecule coding for a protein having the characteristics of a human CSF glycoprotein.
BACKGROUND Colony stimulating factors (CSF) are a group of specific regulatory glycoproteins that stimulate hematopoietic cell proliferation and differentiation.
They are also believed to affect the activity of subpopulations of mature hematopoietic cells which are important in a host defense against infection.
The study of hematopoiesis and the potential role of the hematopoietic cells in the immune response requires the ability to maintain and grow hematopoietic progenitor cells in vitro and in vivo.
Thus CSFs may be employed as reagents for clinical testing and research study of the cells of the hematopoietic and possibly the immune systems.
Several CSFs have been identified which may be useful for these purposes, e.
g.
, CSFs that control granulocyte and macrophage populations, and CSFs that stimulate mast cells and stem cells.
Several human granulocyte and/or macrophage CSFs have presently been identified [See D