Stabilized enzymic solutions for determining urea

DETAILED DESCRIPTION OF THE INVENTION The stabilized enzyme solutions herein can be used in the clinical field for the determination of urea.
The stabilized enzyme solutions herein can be used in conductivimetric methods of analyses or photometric analyses.
The stabilized urease solutions have utility in assays based upon the following reaction sequence: ##STR4## whether the conductivity, rate of change of conductivity or concentration of products (ammonia or carbon dioxide) are determined.
The stabilized urease solutions herein have utility in the enzymic breakdown of urea to products which can be measured and such measurements correlated to urea concentration.
In particular, the stabilized urease solutions have applicability in urea determinations based upon rate of change of conductivity.
When the stabilized enzyme solutions herein are to be used in urea determinations based on photometric methods, a dye or compound absorbing light at a particular wavelength can be added to the solutions herein.
For measuring urea concentration by rate of change of conductivity, the stabilized urease enzyme solutions herein can be used as described.
In the preferred utility of the stabilized urease solutions herein, the stabilized urease solutions can be used in combination with electronic analyzers which measure the conductivity, rate of change of the conductivity and correlate the measured quantities to the concentration of urea in the sample.
Such instruments are commercially available and the solutions herein have especial applicability in such commercially available instruments.
A particularly preferred commercially available instrument is the BUN Analyzer 2, or the "ASTRA.
TM.
" both available from Beckman Instruments, Inc.
Both the "ASTRA.
TM.
" and the BUN Analyzer 2 utilizes the enzymatic conductivimetric rate method employing a conductivity electrode immersed in the sample and an electronics system that measures the rate of increase in conductivity when a precise volume of sample is pipetted into a reaction cup containing the stabilized urea solution