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Stabilized urease composition
| Details |
Inventors: Schneider, Walter; Roder, Albert; Mollering, Hans; Gutmann, Ingeborg;
Assignee: Boehringer Mannheim GmbH (Mannheim-Waldhof, DE)
Primary Examiner: Tanenholtz; Alvin E.
Assistant Examiner:
Attorney, Agent or Firm: Sprung, Felfe, Horn, Lynch & Kramer
Stabilized urease compositions are provided comprising urease and, as a stabilizing agent, a mixture of glutathione, ethylenediamine-tetraacetic acid, and citrate, preferably in a weight ratio of 1:0.5-5:0.5-3:0.5-3. |
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DETAILED DESCRIPTION What is claimed is: 1.
Stabilized urease composition comprising urease of a specific activity of from 25-1500 U/mg and, as a stabilizing agent, a stabilizing mixture of glutathione, ethylenediamine-tetraacetic acid and citrate, wherein said citrate is present in stabilizingly effective amount.
2.
Urease composition a claimed in claim 1, wherein said composition contains urease, glutathione, ethylenediaminetetraacetic acid and citrate in a weight ratio of 1:0.
5-5:0.
5-3:0.
5-3.
3.
Urease composition as claimed in claim wherein said urease is dextran pre-tested urease.
4.
Urease composition as claimed in claim 1 wherein said urease is in lyophilized form.
5.
Urease composition as claimed in claim 1 wherein said urease is in the form of a solution in a glycerol/water mixture.
6.
Reagent for the detection of urea comprising a stabilized urease as claimed in claim 1, and a system for the detection of ammonia.
7.
Reagent as claimed in claim 6 additionally containing a binding agent, carrier and buffer substance.
8.
Reagent as claimed in claim 6 additionally containing a stabilizer for the system for the detection of ammonia.
9.
Reagent as claimed in claim 6 comprising a carrier paper or carrier film impregnated with the stabilized urease and the system for the detection of ammonia or coated on in the form of a binding agent-containing layer.
10.
Reagent as claimed in claim 6 wherein the system for the determination of urea contains at least one pH indicator and optionally a wetting agent and/or buffer.
11.
Reagent as claimed in claim 6 wherein the system for the determination of ammonia comprises glutamic acid dehydrogenase, an alpha-ketoglutarate, reduced nicotinamide-adenine dinucleotide, adenosine diphosphate and a buffer.
12.
Reagent as claimed in claim 6 wherein the system for the determination of ammonia comprises phenol or salicylic acid, sodium nitroferric cyanide and sodium hypochlorite.
13.
Process for preparing a stabilized urease composition which comprises adding to urease of a specific activity of from 25-1500 U/mg, stabilizingly effective amounts of a stabilizing mixture of glutathione, ethylenediamine-tetraacetic acid and citrate, wherein said citrate is present in stabilizingly effective amount
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Molecular Biology 
