Support matrices for immobilized enzymes

DETAILED DESCRIPTION We claim as our invention: 1.
An organic-inorganic matrix having pendent covalent bonding sites for enzymatic conjugates which comprises a porous, water-insoluble, solid inorganic support containing a copolymeric material formed by the in situ reaction product of a water-soluble aminopolystyrene in an aqueous solution possessing a pH of less than 7.
0 and a bifunctional monomer selected from the group consisting of organic compounds containing carbonyl, acyl and isocyanato moieties, wherein said bifunctional monomer is added in a molar excess of 2 to 50 moles of monomer per mol of the amine content of said water-soluble aminopolystyrene.
2.
The matrix as set forth in claim 1 in which said solid support comprises a metallic oxide.
3.
The matrix as set forth in claim 2 in which said metallic oxide is an alumina.
4.
The matrix as set forth in claim 3 in which said alumina is gamma-alumina.
5.
The matrix as set forth in claim 1 in which said solid support is a ceramic monolith coated with a porous metallic oxide.
6.
The matrix as set forth in claim 1 in which said solid support is a porous silica.
7.
The matrix as set forth in claim 1 in which the bifunctional monomer is glutaraldehyde.
8.
The organic-inorganic matrix as set forth in claim 1 which is prepared by depositing a water soluble salt of aminopolystyrene on a solid support from an aqueous solution at a pH less than 7 and thereafter reacting the resultant aminopolystyrene-solid support composite with said bifunctional monomer to form the desired organic-inorganic matrix.
9.
The matrix as set forth in claim 8 in which the pH is in a range of from about 1 to about 4.
10.
The matrix as set forth in claim 8 in which said water soluble salt of aminopolystyrene is the hydrochloric acid salt.
11.
The matrix as set forth in claim 8 in which said water soluble salt of aminopolystyrene is sulfuric acid salt.
12.
The matrix as set forth in claim 8 in which the bifunctional monomer is glutaraldehyde.




Description:
BACKGROUND OF THE INVENTION It is known that enzymes, which are proteinaceous in nature and which are commonly water soluble, comprise biological catalysts which serve to regulate many and varied chemical reactions which occur in living organisms