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Thermally stable and positionally non-specific lipase isolated from Candida
| Details |
Inventors: Ishii, Michiyo;
Assignee: Noro Nordisk A/S (Bagsvaerd, DK)
Primary Examiner: Naff; David M.
Assistant Examiner: Meller; Michael V.
Attorney, Agent or Firm: Zelson; Steve T., Lambiris; Elias J.
Thermally stable, positionally non-specific lipases native to Candida species of C. antartica, C. tsukubaensis, C. auriculariae, C. humicola, and C. foliarum, are isolated. The lipase of C. antarctica, is preferred. Two lipase activities are elaborated by C. antarctica. One lipase fraction being 43 kD in molecular weight, and of an isoelectric point of about 8.0 and has excellent thermostability. The other fraction being 33 kD in molecular weight and of an isoelectric point of about 6.0 and has high retention of residual activity at pH 10. |
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DETAILED DESCRIPTION OF THE INVENTION Microorganisms Lipases according to the invention can be produced by cultivation of strains belonging to the genus Candida, particularly Candida Group I as defined by N.
J.
W.
Kreger van Rij: The Yeasts, a Taxonomic Study third revised and enlarged edition, Elsevier, Amsterdam (1984).
Group I includes the basidiomycetous anamorphs of Candida.
Preferred species are C.
antarctica (Goto et al.
) Kurtzman et al.
, C.
tsukubaensis, C.
auriculariae, C.
humicola or C.
foliarum, as defined in the said book.
It is noted that C.
antarctica has also been described under the synonyms Sporobolomyces antarcticus Goto et al.
, Sterigmatomyces aphidis Henninger & Windisch and Trichosporon oryzae Ito et al.
, and that C.
auriculariae has been described under the synonym Torulopsis auriculariae.
The preferred Candida strains are those that produce non-specific lipase which shows immunochemical identity to one or more of the lipases exemplified hereinafter.
The preferred strains include the following three strains of C.
antarctica, which have been deposited at Deutsche Sammlung von Mikroorganismen (DSM) under the terms of the Budapest Treaty:
______________________________________
Deposit No.
Deposit date
______________________________________
DSM 3855 29 Sep 1986
DSM 3908 8 Dec 1986
DSM 3909 8 Dec 1986
______________________________________
The preferred strains also include the following strains, which are freely available to the public from Centralbureau voor Schimmelculturen (CBS), American Type Culture Collection (ATCC), Agricultural Research Culture Collection (NRRL) and Institute of Fermentation, Osaka (IFO) under the indicated deposit numbers: C.
antarctica: CBS 5955, ATCC 34888, NRRL Y-8295 (type strain) C.
antarctica: CBS 6678, ATCC 28323 C.
antarctica: CBS 6821, NRRL Y-7954, ATCC 32657 C.
tsukubaensis: CBS 6389,IFO 1940, ATCC 24555, NRRL Y-7792 (type strain) C.
auriculariae: CBS 6379, ATCC 24121, IFO 1580 (type strain) C
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Molecular Biology 
