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 Transport medium for microorganisms

Details
Inventors: Bryan, Lawrence E.;
Assignee: Her Majesty the Queen in right of Canada, as represented by the Minister (Ottawa, CA)
Primary Examiner:
Assistant Examiner:
Attorney, Agent or Firm:

The invention disclosed is an improved transport medium for microorganisms, the improvement residing in the inclusion of Mg.sup.++ in a concentration of 0.6 to 1.2 g per liter of medium, preferably about 1.0 g/l. Further improvement resides in the timing of inclusion of the reducing agent, sodium thioglycollate, i.e. just before use. It has also been found that the detoxification agent, activated charcoal, may be included in concentrations of 5-10 g/l of medium.

DETAILED DESCRIPTION The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows: 1.
A transport medium for microorganisms, which comprises
______________________________________
Agar (purified) 3-4 g
NaCl 10 g
KCl 0.
2 g
K.
sub.
2 HPO.
sub.
4 1.
39 g
KH.
sub.
2 PO.
sub.
4 0.
2 g
CaCl.
sub.
2.
2H.
sub.
2 O 0.
1 g
Mg.
sup.
++ 0.
6-1.
2 g
Activated Charcoal 5-10 g
______________________________________
and distilled water to a final volume of 1 liter.
2.
A transport medium according to claim 1, which comprises about 1.
0 g of Mg.
sup.
++ per liter of transport medium.
3.
A transport medium according to claim 2, which further comprises 10 g of activated charcoal per liter of transport medium.
4.
A transport medium according to claim 1, which further comprises about 0.
1% sodium thioglycollate.
5.
A transport medium according to claim 1, which further comprises about 20% glycerol.
6.
A transport medium according to claim 1, wherein the pH of the medium is in the range of 6.
5-6.
9.
7.
A transport medium for micro-organisms, which comprises
______________________________________
Agar 3 g
NaCl 10 g
KCl 0.
2 g
K.
sub.
2 HPO.
sub.
4 1.
39 g
KH.
sub.
2 PO.
sub.
4 0.
2 g
CaCl.
sub.
2.
2H.
sub.
2 O 0.
1 g
MgCl.
sub.
2.
6H.
sub.
2 O 10.
17 g
Activated Charcoal 10 g
______________________________________
and water to a final volume of 1 liter.
8.
A transport medium according to claim 7, which further comprises about 0.
1% sodium thioglycollate.
9.
A transport medium according to claim 7, which further comprises about 20% glycerol.
10.
A transport medium according to claim 7, wherein the pH of the medium is in the range of 6.
5-6.
9.




Description:
This invention relates to a transport medium for micro-organisms and in particular to a universal transport medium for supporting the viability of a wide spectrum of micro-organisms during transport from the field to a remote laboratory for testing



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