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Viscosity Probe
| Details |
Inventors: Darling, Graham D.; Heisler, deceased, Seymour; Stranix, Brent R.; Turkewitsch, Petra; Wandelt, Barbara;
Assignee: McGill University (CA)
Primary Examiner: Leary; Louise
Assistant Examiner:
Attorney, Agent or Firm: Bachman & LaPointe, P.C.
Substituted aminostyrylpyridinium salts are fluorescent dyes having advantageous characteristics when employed as viscosity probes for the measurement of viscosity in a liquid, especially in a very small volume, for example, in living cells. |
|
DETAILED DESCRIPTION We claim: 1.
A substituted aminostyrylpyridinium salt of formula (I): ##STR7## wherein Y.
sub.
1 is ##STR8## and Y.
sub.
2 is H; R.
sub.
1 and R.
sub.
2, which are the same or different are straight or branched chain lower alkyl of 1 to 6 carbon atoms or R.
sub.
1 and R.
sub.
2 together with the N atom to which they are attached form a cyclic ring of 5 or 6 ring atoms; one of Z and Y is >N.
sup.
+ --R X.
sup.
- and the other is CH; R is selected from the group consisting of (a) --(CH.
sub.
2).
sub.
m COOR.
sub.
4 in which m is an integer of 1 to 10 and R.
sub.
4 is a straight or branched chain alkyl of 3 to 18 carbon atoms, aryl of 6 to 14 carbon atoms, aralkyl in which the alkylene moiety has 1 to 18 carbon atoms and the aryl moiety has 6 to 14 carbon atoms, said aryl and aryl moiety being unsubstituted or substituted one or more times by one or more of nitro, fluoro, chloro, bromo, iodo and carboxylate; (b) --(CH.
sub.
2).
sub.
p ArCOO.
sup.
- in which p is 0 to 15, Ar is arylene of 6 to 14 carbon atoms unsubstituted or substituted one or more times by one or more of nitro, fluoro, chloro, bromo, iodo, carboxylate or --COOR.
sub.
4 in which R.
sub.
4 is as defined above, and (c) --(CH.
sub.
2).
sub.
p ArCOOR.
sup.
4 in which p, Ar and R.
sup.
4 are as defined above, and X.
sup.
- is selected from the group consisting of chloride, bromide, iodide, toslylate, mesylate and brosylate.
2.
The salt according to claim 1, being a dialkylaminostyrylpyridinium salt of formula (II): ##STR9## wherein R.
sub.
1 and R.
sub.
2, which are the same or different are straight or branched chain lower alkyl of 1 to 6 carbons; R is --(CH.
sub.
2).
sub.
m COOR.
sub.
4 in which m is an integer of 1 to 10 and R.
sub.
4 is a straight or branched chain alkyl of 3 of 6 carbon atoms; and X.
sup.
- is a halide ion selected from the group consisting of chloride, bromide and iodide.
3.
The salt according to claim 2, in which R.
sub.
1 and R.
sub.
2 are both methyl, R is --CH.
sub.
2 COOBu, in which Bu is n-butyl and X.
sup.
- is chloride.
Description:
BACKGROUND OF THE INVENTION i) Field of the Invention This invention relates to substituted aminostyrylpyridinium salts and their use as viscosity probes for measurement of the viscosity of a liquid
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Molecular Biology 
