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.alpha.-Ketoglutarate dehydrogenase gene
| Details |
Inventors: Asakura, Yoko; Usuda, Yoshihiro; Tsujimoto, Nobuharu; Kimura, Eiichiro; Abe, Chizu; Kawahara, Yoshio; Nakamatsu, Tsuyoshi; Kurahashi, Osamu;
Assignee: Ajinomoto Co., Inc. (Tokyo, JP)
Primary Examiner: Jones; W. Gary
Assistant Examiner: Whisenant; Ethan
Attorney, Agent or Firm: Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity. |
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DETAILED DESCRIPTION What is claimed is: 1.
An isolated gene comprising 1) a nucleotide sequence which codes for an enzyme having the amino acid sequence shown in SEQ ID NO: 2 or 2) a nucleotide sequence which codes for an enzyme having substitution, deletion, or insertion of one or more amino acid residues in the amino acid sequence shown in SEQ ID NO: 2 and increases .
alpha.
-ketoglutarate dehydrogenase activity to produce succinyl-CoA from .
alpha.
-ketoglutarate in a coryneform L-glutamic acid-producing bacterium when alone introduced into the coryneform L-glutamic acid producing bacterium.
2.
The gene according to claim 1, wherein the enzyme has the amino acid sequence shown in SEQ ID NO: 2.
3.
A recombinant DNA obtained by ligating a gene comprising a nucleotide sequence which codes for an enzyme having the amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence having substitution, deletion, or insertion of one or more amino acid residues in the amino acid sequence shown in SEQ ID NO: 2 and increases .
alpha.
-ketoglutarate dehydrogenase activity to produce succinyl-CoA from .
alpha.
-ketoglutarate in a coryneform L-glutamic acid-producing bacterium when alone introduced into the coryneform L-glutamic acid-producing bacterium, with a vector which functions in coryneform bacteria.
4.
A coryneform bacterium harboring the recombinant DNA according to claim 3.
5.
A method of producing L-lysine comprising the steps of cultivating a coryneform bacterium harboring the recombinant DNA according to claim 3 and having L-lysine productivity in a liquid medium, to allow L-lysine to be produced and accumulated in a culture liquid, and collecting the L-lysine.
6.
A recombinant DNA according to claim 3, wherein said enzyme has the amino acid sequence shown in SEQ ID NO: 2.
7.
The coryneform bacterium according to claim 4, wherein said enzyme has the amino acid sequence shown in SEQ ID NO:2.
8.
The method according to claim 5, wherein said enzyme has the amino acid sequence shown in SEQ ID NO: 2
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Molecular Biology 
