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Substitute blood vessel and a process for preparing the same
| Details |
Inventors: Miyata, Kaneyasu; Amano, Izumi; Iwai, Takehiko; Asahi, Tetsuya;
Assignee: Miyata; Kaneyasu (Nagoya, JP); Amano; Izumi (Nagoya, JP); Toda; Kohei (Osaka, JP)
Primary Examiner: Kight, III; John
Assistant Examiner:
Attorney, Agent or Firm: Shlesinger, Arkwright, Garvey & Dinsmore
A process for preparing a heterograft substitute blood vessel comprises digesting a properly pretreated pig blood vessel with a digesting solution containing an animal proteolytic enzyme so as to retain collagenous and elastic fiber constituents substantially in the original state and substantially remove away other tissue constituents of said blood vessel than said two kinds of constituents, and then fixing the resulting digested blood vessel into a desired shape with a liquid fixing agent. |
|
DETAILED DESCRIPTION What is claimed is: 1.
A process for preparing a heterograft substitute blood vessel comprising digesting a properly pretreated pig blood vessel with a digesting solution containing an animal proteolytic enzyme so as to retain collagenous and elastic fiber constituents substantially in the original state and substantially remove away other tissue constituents of said blood vessel than said two kinds of constituents, and then fixing the resulting digested blood vessel into a desired shape with a liquid fixing agent.
2.
A process as claimed in claim 1, in which said animal proteolytic enzyme is at least one selected from a group consisting of pepsin, trypsin and chymotrypsin.
3.
A process as claimed in claim 1, in which said pig blood vessel is a raw or a protein denaturated blood vessel obtained by freeze-preservation.
4.
A process as claimed in claim 1, in which said liquid fixing agent comprises 5 - 10% formalin solution or 2 - 25% glutaric aldehyde solution.
5.
A process as claimed in claim 1, in which said digesting solution is a mixed buffer solution containing an animal proteolytic enzyme, a thrombus dissolving agent and an anticoagulating agent.
6.
A process as claimed in claim 1, in which said digesting treatment is performed at pH 1.
8 - 9.
0, at 30.
degree.
- 40.
degree.
C.
for 2 - 8 hours.
7.
A process as claimed in claim 1, in which said digesting treatment is controlled so that extension percentage of the resulting heterograft substitute blood vessel is more than 40% under 200 g load and its tensile strength more than 1.
0 kg.
8.
A process as claimed in claim 1, in which said digesting treatment comprises digesting a raw pig blood vessel with a digesting solution containing about 100 U/ml chymotrypsin at pH 6 - 8 and at 30.
degree.
- 40.
degree.
C.
for 3 - 8 hours.
9.
A process as claimed in claim 1, in which said digesting treatment comprises digesting a raw pig blood vessel with a digesting solution containing about 1% trypsin at pH 6 - 8 and at 30.
degree.
- 40
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