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Analysis element and process for its manufacture
| Details |
Inventors: Deeg, Rolf; Maurer, Eberhard; Klose, Sigmar; Kopfer, Bernhard; Babiel, Reiner;
Assignee: Boehringer Mannheim GmbH (Mannheim, DE)
Primary Examiner: Scheiner; Toni R.
Assistant Examiner: Chin; Christopher L.
Attorney, Agent or Firm: Nikaido, Marmelstein, Murray & Oram
An analysis element for the determination of an analyte in a liquid sample, especially for medicinal uses. A carrier layer (2) contains, in a reagent domain (4), a reagent applied in a defined pattern by an ink-jet process. The pattern comprises several sets (A, B, C) of compartments (11-20), the compartments (e.g. 11, 13, 15, 17, 19) of the same set (e.g. A) having the same chemical composition, the compartments (11, 13, 15, 17, 19 or 12, 16, 20) of different sets (A or B) containing different reagents and the compartments of different sets being arranged in alteration so that the compartments containing different reagents are close together but nevertheless spatially separated. |
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DETAILED DESCRIPTION According to the invention, an analysis element of the type indicated at the outset is characterized in that the pattern applied to the reagent domain comprises several sets of compartments, the compartments of the same set having the same chemical composition, the compartments of different sets containing different regents and the compartments of different sets being arranged in alteration so that the compartments containing different reagents are close together but nevertheless spatially separated.
The average distance between the outer limits of the compartments of different sets is typically less than 1 mm and preferably less than 0.
5 mm.
The combination of the measures according to the invention is also denoted as "microcompartmentalization" hereafter.
The term "compartment" denotes a delimited sub-domain.
A compartment can consist of one dot or several mutually overlapping dots.
The compartments which contain different reagents (and thus belong to different sets of compartments in the reagent domain) are at least in some cases spatially separated from one another in that they are arranged next to one another (although not necessarily in the same plane) in the reagent domain, the compartments containing different reagents being alternate, i.
e.
compartments of different sets being alternately adjacent.
For practical reasons, it is convenient to have regular alteration where, for example, the compartments of three sets A, B and C are present in a cyclically repeating pattern A, B, C, A, B, C, A, B etc.
In exceptional cases, however, it may also be convenient to have an alternating pattern without cyclic repetition.
The compartments can also be arranged on top of one another in some cases.
A spatial separation of the compartments in the direction perpendicular to their planar dimension (vertical compartmentalization) is possible here if an isolating intermediate layer consisting of a soluble inert isolating substance (e.
g.
an inert protein or film former) is applied
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