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Home Semiconductor manufacture Silicon-tetrahydrazide-for-purification-of-DNA

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 Silicon tetrahydrazide for purification of DNA

Details
Inventors: Woodard, Daniel L.; Howard, Adriann J.;
Assignee: Becton, Dickinson and Company (Franklin Lakes, NJ)
Primary Examiner: Kunz; Gary L.
Assistant Examiner:
Attorney, Agent or Firm: Fugit; Donna R.

Silicon tetrahydrazide compounds produced by reacting SiCl.sub.4 with hydrazine to completion. The compounds are useful for binding DNA, as they do not require the use of chaotrope or alcohol solutions for binding. Binding and elution of DNA can be accomplished in low salt buffers or water. Kits containing the compounds for purification of DNA are also disclosed.

DETAILED DESCRIPTION The invention provides the monomer unit composition: ##STR3## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H, R is HN--NH.
sub.
2, and X is HN--NH.
sub.
2, and repeating units of the composition comprising the formula: ##STR4## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H or a monomer unit, R is HN--NH.
sub.
2 or a monomer unit, X is HN--NH.
sub.
2 or a monomer unit, and compositions comprising the above monomer and repeating units thereof.
Repeating units described above can include from about 2 up to infinity.
Ranges include 2 to 100,000,000, and 2 to 100,000.
The surface provides for bonding of DNA while also allowing easy recovery of DNA from the surface.
Also provided is a process for purifying DNA which comprises contacting DNA with a composition of the formula: PATENT ##STR5## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H, R is HN--NH.
sub.
2, and X is HN--NH.
sub.
2, and repeating units of the composition comprising the formula: ##STR6## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H or a monomer unit, R is HN--NH.
sub.
2 or a monomer unit, X is HN--NH.
sub.
2 or a monomer unit, and compositions comprising the above monomer and repeating units thereof.
Reaction products of H2NNH.
sub.
2 and SiCl.
sub.
4 are also provided.
The invention also provides a method for making the composition of the formula: ##STR7## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H, R is HN--NH.
sub.
2, and X is HN--NH.
sub.
2, and repeating units of the composition comprising the formula: ##STR8## wherein T is HN--NH.
sub.
2 or Si(OH).
sub.
3, Y is H or a monomer unit, R is HN--NH.
sub.
2 or a monomer unit, X is HN--NH.
sub.
2 or a monomer unit.
Repeating units described above can include from about 2 up to infinity.
Ranges include 2 to 100,000,000, and 2 to 100,000.
Generally, reaction products of H.
sub.
2 NNH.
sub.
2 and SiCl.
sub.
4 result in a bead like structure comprising repeating units of the above referenced monomer unit.
It is possible that the electronic nature of this polymer is such that surface modifications can be made that are of a more conventional nature but are changed electronically due to the presence of this polymer being at the center of the bead (making it a more efficient surface for the purposes described in this disclosure)



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