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Staining with nitrido-bridged osmium or ruthenium compounds
| Details |
Inventors: Cleare, Michael J.; Hydes, Paul C.;
Assignee: Johnson, Matthey & Co., Limited (London, GB2)
Primary Examiner: Fagelson; Anna P.
Assistant Examiner:
Attorney, Agent or Firm: Cushman, Darby & Cushman
This invention relates to the staining of cells, and in particular provides a novel reagent for the detection, for example, of DNA (deoxyribonucleic acid) and polysaccharides by electron microscopy. A reagent for staining cells in accordance with the present invention comprises an aqueous solution of a nitrido-bridged complex of osmium or ruthenium and having the general formula: [M.sub.2.sup.IV N(NH.sub.3).sub.8-2x X.sub.5-y (H.sub.2 O).sub.x ]Y.sub.y. where M represents either Os or Ru, X and Y are the same or different anions selected from nitrate, halide and pseudohalide, x is 0 or 1 and y is 2 or 3. |
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DETAILED DESCRIPTION We claim: 1.
A method for staining cells of a prepared biological specimen which comprises submitting said specimen firstly to mild acid hydrolysis and secondly to reaction with a staining reagent comprising an aqueous solution of a nitrido-bridged complex of osmium or ruthenium having the general formula [M.
sub.
2.
sup.
IV N(NH.
sub.
3).
sub.
8-2x X.
sub.
5-y (H.
sub.
2 O).
sub.
x ]Y.
sub.
y where M represents either Os or Ru, X and Y are the same or different anions selected from the group consisting of nitrate, halide and psuedohalide, x is 0 or 1 and y is 2 or 3.
2.
A method according to claim 1 wherein the said complex has the formula (i) [Os.
sub.
2 N(NH.
sub.
3).
sub.
8 X.
sub.
2 ]Y.
sub.
3, (ii) [Ru.
sub.
2 N(NH.
sub.
3).
sub.
8 X.
sub.
2 ]Y.
sub.
3, or (iii) [Ru.
sub.
2 N(NH.
sub.
3).
sub.
6 X.
sub.
3 (H.
sub.
2 O)]Y.
sub.
2.
3.
A method according to claim 2 wherein X and Y in formula (i) and formula (ii) are chloride.
4.
A method according to claim 2 wherein the complex has the formula: (iv) [Os.
sub.
2 N(NH.
sub.
3).
sub.
8 Cl.
sub.
2 ]Cl.
sub.
3, (v) [Ru.
sub.
2 N(NH.
sub.
3).
sub.
8 Cl.
sub.
2 ]Cl.
sub.
3, (vi) [Ru.
sub.
2 N(NH.
sub.
3).
sub.
6 Cl.
sub.
3 (H.
sub.
2 O)]Cl.
sub.
3, or (vii) [Ru.
sub.
2 N(NH.
sub.
3).
sub.
8 Br.
sub.
2 ]Br.
sub.
3.
Description:
This invention relates to the staining of cells, and in particular provides a novel reagent for the detection, for example, of DNA (deoxyribonucleic acid) and polysaccharides by electron microscopy.
In the technique of electron microscopy, previously-prepared biological specimens are commonly reacted with a staining reagent in order to increase the differential electron scattering power of the specimen constituents.
In other words, the contrast of the electron micrograph of the specimen is increased.
Particular reagents are known to stain particular compounds or classes of compound; for example, phosphotungstic acid is a cationic stain and tends to bind to proteins, whereas uranyl acetate is an anionic stain and is reasonably selective for nucleic acids and, to a lesser extent, proteins
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Semiconductor manufacture 
