 Composition for suppressing appetite |
| OF THE PREFERRED EMBODIMENTS The compositions of the invention comprise kavalactones and ... |
|
| Methods and materials for treating depression and mood disorder |
| Treatment of depression or mood disorder depends largely on a few central elements. Because mental ... |
|
| Methods and apparatus for fine particle formation |
| OF THE PREFERRED EMBODIMENTS A preferred embodiment of the present invention is shown in FIG. 1 ... |
|
| Sore throat spray |
| What is claimed is: 1. A throat spray for topical application to a sore throat, said throat spray ... |
|
| Over-coated chewing gum formulations including tableted center |
| OF THE PRESENTLY PREFERRED EMBODIMENTS The present invention provides improved methods for ... |
|
| Method to enhance innate immunity defense mechanisms by treatment with plant-derived alkaloids |
| OF THE INVENTION The most important function of the immune system is to provide its host with ... |
|
| Thermogenic weight management composition |
| OF THE INVENTION The present invention involves a composition for oral ingestion that contains an ... |
|
| Composition for domestic livestock grafting and method of making and using |
| I claim: 1. A method of facilitating acceptance of a juvenile animal by a prospective mother, the ... |
|
| Method of treating skin virus infections |
| The invention accordingly relates to a material which provides an antioxidant effect when applied ... |
|
|
Method for immobilizing biomolecules and affinity ligands on polymer carriers
| Details |
Inventors: Glusenkamp, Karl-Heinz; Eberle-Adamkiewics, Gertrud;
Assignee:
Primary Examiner: Baker; Maurie
Assistant Examiner:
Attorney, Agent or Firm: R W Becker & Associates, Becker; R W
In a method for immobilizing biomolecules and affinity ligands water insoluble matrices, having amino groups and selected from test tubes, microtiter plates, microscope slides, beads, membranes, resins, and filters, are reacted with a cyclobutene carboxylic acid derivative, such as cyclobutene carboxylic acid diester, cyclobutene carboxylic acid halide, cyclobutene carboxylic acid ester halide, cyclobutene carboxylic acid dialkoxyester, and cyclobutene carboxylic acid imidazole, as an activating compound in methanol and triethylamine to form active matrices with active groups. A protein containing at least one primary or secondary amino group, is dissolved and added to the matrices. The activated matrices and the dissolved protein are incubated at pH of 7-10 and a temperature of +4.degree. C. to +60.degree. C. in an aqueous buffer system, free of primary and secondary amines, to thereby immobilize the protein on the matrices. |
|
DETAILED DESCRIPTION What is claimed is: 1.
A method for immobilizing biomolecules and affinity ligands, said method comprising the steps of: a) reacting water insoluble matrices, having amino groups and being selected from the group consisting of test tubes, microtiter plates, microscope slides, beads, membranes, resins, and filters, with a cyclobutene carboxylic acid derivative, selected from the group consisting of cyclobutene carboxylic acid diester, cyclobutene carboxylic acid halide, cyclobutene carboxylic acid ester halide, cyclobutene carboxylic acid dialkoxyester, and cyclobutene carboxylic acid imidazole, as an activating compound in methanol in the presence of triethylamine to form active matrices with active groups; b) dissolving a protein containing at least one primary or secondary amino group and adding the protein to the activated matrices; c) incubating the activated matrices and the protein of step b) at a pH of 7-10 and a temperature of +4.
degree.
C.
to +60.
degree.
C.
in an aqueous buffer system, free of primary and secondary amines, to thereby immobilize the protein of step b) on the matrices.
2.
A method according to claim 1, further including the steps of: d) reacting the matrices of step c) with a cyclobutene carboxylic acid derivative, selected from the group consisting of cyclobutene carboxylic acid diester, cyclobutene carboxylic acid halide, cyclobutene carboxylic acid ester halide, cyclobutene carboxylic acid dialkoxyester, and cyclobutene carboxylic acid imidazole, as an activating compound in methanol in the presence of triethylamine to reactivate the matrices with active groups; e) dissolving a protein containing at least one primary or secondary amino group and adding the protein to the reactivated matrices of step d); f) incubating the reactivated matrices and the protein of step e) at a pH of 7-10 and a temperature of +4.
degree.
C.
to +60.
degree.
C.
in an aqueous buffer system, free of primary and secondary amines, to thereby immobilize the compound of step e) at the active groups of the reactivated matrices
|
|
Weight Loss and Supplements 
