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| Sore throat spray |
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| Over-coated chewing gum formulations including tableted center |
| OF THE PRESENTLY PREFERRED EMBODIMENTS The present invention provides improved methods for ... |
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| Method to enhance innate immunity defense mechanisms by treatment with plant-derived alkaloids |
| OF THE INVENTION The most important function of the immune system is to provide its host with ... |
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| Thermogenic weight management composition |
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| Composition for domestic livestock grafting and method of making and using |
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Process for separating milk clotting enzymes, and stable rennet compositions
| Details |
Inventors: Larsen, Robert; Harboe, Marianne Kirsten; Ladsten, P.ang.l Martin;
Assignee: CHR. Hansen A/S (DK)
Primary Examiner: Tsang; Cecilia J.
Assistant Examiner: Gupta; Anish
Attorney, Agent or Firm: Hunton & Williams
A process of separating milk clotting enzymes in extracts of animal stomach tissue which comprises contacting a partially purified extract with an ion exchange resin under conditions where chymosin is bound to the resin and recovering the chymosin, the process optionally comprising the further step of recovering pepsin, from a pepsin-rich extract fraction using an ion exchange resin, and a liquid of powdered rennet composition containing at least 90% of its milk clotting activity as chymosin activity, the composition comprising a chymosin stabilizing agent which is typically selected from a protein, a peptide, an amino acid or ascorbic acid. |
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DETAILED DESCRIPTION We claim: 1.
A process of separating milk clotting aspartic endopeptidases present in an extract of animal stomach tissue, comprising the steps of (i) preparing an aqueous extract of animal stomach tissue that contains the aspartic endopeptidases chymosin and pepsin, and/or their pro-enzymes, to obtain a one-phase, crude and particulate matter-containing extract comprising said endopeptidases, (ii) adjusting the pH in the extract to a value where the pro-enzymes are converted into active endopeptidases, and keeping the extract at this pH until the pro-enzymes are activated, (iii) separating particulate matter from the extract resulting from step (ii) to obtain a one-phase, partially purified aqueous extract containing the milk clotting endopeptidases essentially in their active forms, (iv) mixing the partially purified extract of step (iii) with an ion exchange resin under conditions where pseudochymosin and chymosin present in the extract, but not the pepsin also present, is bound to the resin, (v) separating the mixture of (iv) into the ion exchange resin with chymosin and pseudochymosin bound thereto and an extract fraction containing the pepsin also present in the partially purified extract resulting from step (iii), (vi) recovering, under conditions where essentially all pseudochymosin is converted into chymosin, the chymosin and pseudochymosin from the ion exchange resin into an aqueous medium to obtain a solution containing the chymosin and pseudochymosin present in the partially purified extract resulting from step (iii) as chymosin.
2.
A process according to claim 1 wherein pH is adjusted in step (ii) to a value which is in the range of 0.
5 to 5.
0.
3.
A process according to claim 2 wherein the pH is adjusted to a value which is in the range of 1.
0 to 3.
0.
4.
A process according to claim 3 wherein the pH is adjusted to a value which is in the range of 1.
5 to 2.
5.
5.
A process according to claim 1 wherein particulate matter is separated in step (iii) by filtration or centrifugation
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